Fig. 2: Identification of an HPV18E784-98-specific TCR restricted to HLA-DRA/DRB1*09:01.
a Epitope identification of 10F04 TCR. Series truncated peptides derived from HPV18E776-105 were pulsed on autologous LCLs to stimulate 10F04 transduced T cells. The concentration of IFNγ in the supernatant after overnight co-culture was detected by ELISA. b HLA restriction analysis of 10F04. The 10F04 transduced T cells were co-cultured with autologous LCLs pulsed with the HPV18E776-105 peptide in the presence of blocking antibodies or isotype control. The IFNγ secretion level was detected by ELISA. c 10F04 transduced T cells were co-cultured with HEK-293T cells expressing each HLA-II molecule of the autologous LCL, which were pulsed with HPV18E776-105 peptide. Autologous LCLs pulsed with HPV18E776-105 peptide were used as a positive control. IFNγ secretion in the supernatant after overnight co-culture was detected by ELISA. d Avidity assay of 10F04 TCR transduced T cells. Serial diluted HPV18E776-105 peptide were used to assess the binding avidity of 10F04 and P09B08. IFNγ secretion was detected by ELISA. e Specific recognition analysis of 10F04 transduced T cells against HPV18E7+ cervical cancer cells. CaSki, and HeLa cell lines were incubated with 10F04 TCR-T cells and recognition was determined by the IFNγ ELISA analysis of the supernatant. HPV18E784-98 peptide pulsed HLA-DRA/DRB1*0901 positive LCLs were set up as positive control. Data are shown as the mean ± SD, n = 3 biologically independent samples (a–e). Source data are provided as a Source Data file.
