Fig. 1: CWC15 is required for miRNA accumulation.
From: The spliceosome-associated protein CWC15 promotes miRNA biogenesis in Arabidopsis
a Diagram of the artificial miRNA targeting CWC15 (amiRCWC15). Solid line represents Watson–Crick pairing and a “0” indicates a C-U mismatch pair. CDS: coding sequence; 5’UTR: 5’ untranslated region; 3’UTR: 3’ untranslated region. b Seedlings of Col and amiRCWC15 (Scale bar: 1 cm). c miRNA levels in various genotypes detected by Northern blot. U6 RNA serves as the loading control. The numbers shown below the pictures indicate the relative amounts of miRNAs (the values in Col were set as 1). d miRNA levels in various genotypes detected by RT-qPCR. miRNA levels were normalized to U6. Error bars indicate SD from three biological replicates (n = 3) and data are presented as mean values +/- SD. Asterisks indicate significantly reduced expression of amiRCWC15 compared to Col based on P-values (two-tailed unpaired t-test) (P < 0.01): miR156a, 5.52677E-06; miR167a, 0.000834; miR173, 3.13908E-05; miR159a, 6.66758E-06; miR167c, 6.62854E-06; miR166a, 0.001156458. e Small RNA sequencing analysis in Col and amiRCWC15−1. The miRNA abundance was calculated as reads per million, and a Log2-transformed ratio of amiRCWC15/Col-0 was plotted. Each circle represents one miRNA. N = 375 distinct miRNAs. f The transcript levels of miRNA target genes in various genotypes detected by RT-qPCR. The levels of miRNA target transcripts were normalized to those of ACTIN2 and compared with Col. Error bars indicate SD from three biological replicates (n = 3) and data are presented as mean values +/- SD. Asterisks indicate significantly increased expression of amiRCWC15 −1/−2 compared to Col based on P-values (two-tailed unpaired t-test) (P < 0.05): CMT3, 0.0193/0.0164; PHV, 0.00293/0.0423; SPL3, 0.00243/0.00273; SPL9, −.00409/0.0738; CKB3, 0.00027/0.0532; APS3, 0.002/0.000183; ARF8, 0.000591/0.0011.Source data are provided as a Source Data file.
