Fig. 1: Three closely related RLKs, including ARH1, FEI1, and FEI2, negatively regulate root hydrotropism. | Nature Communications

Fig. 1: Three closely related RLKs, including ARH1, FEI1, and FEI2, negatively regulate root hydrotropism.

From: Defects in the cell wall and its deposition caused by loss-of-function of three RLKs alter root hydrotropism in Arabidopsis thaliana

Fig. 1

a–c Transgenic plants overexpressing ARH1, FEI1, or FEI2 show decreased root hydrotropic response. d Measurements of root hydrotropic curvatures of Col-0, 35 S::ARH1, 35 S::FEI1, and 35 S::FEI2 transgenic seedlings. e–g Root hydrotropic response of wild-type (Col-0) and the triple mutant (arh1-2 fei1-C fei2-C). h Measurements of root hydrotropic curvatures. i–n Propidium iodide (PI)-stained root tips of Col-0 and the triple mutant after 2-h hydrostimulation treatments. o Cortex cell number differences of right side versus left side (control) for Col-0 and the triple mutant as represented in (i, l), and lower water potential side versus higher water potential side (hydrostimulation) as represented in (j) and (m) within a 200 μm meristematic zone starting from the quiescent center. Red arrow heads in (i, j, l, m) indicate the borders of 200 μm meristem zone starting from the quiescent center. Boxplots span the first to the third quartiles of the data, and whiskers indicate the minimum and maximum values. The line in the box represents the mean. Each circle represents the measurement of an individual root tip (d, h, o). “n” represents the number of roots analyzed in the experiment. Three independent biological replicates were carried out. Scale bars represent 10 mm in (a–c, e–g) and 100 µm in (i–n). One-way ANOVA with Tukey’s multiple comparison test was used for statistical analyses with P < 0.01.

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