Fig. 4: ARFTF17 interacts with MYB40 to regulate pericarp development.
From: An ARF gene mutation creates flint kernel architecture in dent maize
a BiFC assay showing interaction between ARFTF17 and MYB40 in tobacco leaf protoplasts. Scale bars, 20 μm. b LIC assay showing interaction between ARFTF17 and MYB40 in a tobacco leaf. c In vitro pull-down assay showing interaction between ARFTF17 and MYB40. d The kernel phenotype of B73 and aMYB40 overexpression line (MYB40-OE1). Scale bar, 1 cm. (e) Longitudinal-sections of mature kernels of B73 and MYB40-OE1. Scale bar, 2 mm. f RT-qPCR analysis of MYB40 in pericarps of B73 and MYB40-OE1. g, h Dent degree of the kernel crown (g) and pericarp length (h) of B73 and MYB40-OE1 at maturity. Data are mean ± s.d. (n = 20 kernels from 6 ears). i The kernel phenotype of B73, fka1-1, MYB40RNAi and fka1-1;MYB40RNAi. Scale bar, 1 cm. j Longitudinal-sections of mature kernels of B73, fka1-1, MYB40RNAi and fka1-1;MYB40RNAi. Scale bar, 2 mm. k, l RT-qPCR analysis of MYB40 (k) and ARFTF17 (l) in pericarps of B73, fka1-1, MYB40RNAi and fka1-1;MYB40RNAi. m, n Dent degree (m) and pericarp length (n) of kernels of B73, fka1-1, MYB40RNAi and fka1-1;MYB40RNAi at maturity. Data are mean ± s.d. (n = 20 kernels from 6 ears). In (f, k, l), expression levels were normalized to that of TUA4 (Zm00001d013367). Data are mean ± s.d. (n = 3 biologically independent samples). Two-tailed Student’s t tests were used to determine P values shown in the (j, h, m, n).
