Fig. 4: CAT-S-guided discovery of “mito orphans”.
From: Bioorthogonal photocatalytic proximity labeling in primary living samples

a Visualization of CAT-S datasets including log2(+/− catalyst) values of individual experiments in two dimensions by t-SNE analysis, for combined (HeLa, HEK293T, and K562) datasets. Proteins are colored based on their prior annotations in the database. Red, mitochondrial localization. Purple, MAM (mitochondria-associated membrane) or ER-mitochondria contact site localization. Proteins without such annotation but clustered with mitochondrial proteins are potential “mito orphans”. The validated “mito orphans” are denoted. b Imaging of overexpressed orphan proteins in HEK293T cells. Green, staining of mitochondrial marker AIF. Red, staining of overexpressed target protein by C-terminal V5 tag. Blue, staining of the nucleus by Hoechst 33342. Scale bar, 5 µm. c Quantification of mitochondrial colocalization (Pearson’s R value) for “mito orphans” and controls. Data are represented as mean ± SD (n = 12 cells). Source data are provided as a Source Data file.