Fig. 3: The bead-chain-like structure of the full-length neck fiber.
From: Structure of the intact tail machine of Anabaena myophage A-1(L)
a The negative-staining EM image of A-1(L) particles. The red arrows indicate the five neck fibers protruding from one A-(L) particle. b Gel filtration chromatography profile of the recombinant neck fibers and SDS-PAGE analysis of the highest peak fraction. c Negative-staining EM image of the recombinant neck fibers. The cyan arrows indicate the terminal beads with a larger size. d SEC-MALS profile of the recombinant neck fibers. The eluted peak corresponds to the X axis. The black line represents the UV absorbance at 280 nm (Y-axis on the left), whereas the jagged short red line indicates the molecular weight (Y-axis on the right). The dashed line indicates the predicted molecular weight of the highest peak fraction. e The modeled structure of the full-length neck fiber. The gp80, gp81, and gp82 are colored in green, blue, and pink, respectively. Gel filtration chromatography profiles of the recombinant eGFP-gp82-gp81 (f) and gp81-gp80 (g) complexes. The corresponding negative-staining EM images of the highest peak fraction were also displayed in the upper right corner. Each experiment in this figure was repeated independently at least three times with similar results.
