Fig. 1: Identification of obesity-induced upregulation of TM4SF19 in adipose tissue macrophages. | Nature Communications

Fig. 1: Identification of obesity-induced upregulation of TM4SF19 in adipose tissue macrophages.

From: TM4SF19-mediated control of lysosomal activity in macrophages contributes to obesity-induced inflammation and metabolic dysfunction

Fig. 1

A Venn diagrams illustrating overlaps between differentially expressed genes (DEGs: fold change >2, p-value < 0.05) from three independent datasets (GSE182930: Transcriptomics profiling of gonadal white adipose tissue (GWAT) of WT mice after normal chow diet (NCD) and high-fat diet (HFD) feeding for 8 weeks, GSE150102: Transcriptomics profiling of GWAT of WT and leptin-deficient mice, GSE59034: Transcriptomics profiling of human subcutaneous WAT of lean and obese subjects). B Heatmap of 17 upregulated genes identified in (A). C Tm4sf19 mRNA expression levels in various tissues and bone marrow-derived macrophages (BMDMs) of mice after 12 weeks of NCD and HFD feeding (n = 6 mice). D Immunoblot analysis of TM4SF19 in GWAT of mice after 12 weeks of NCD and HFD feeding (n = 6 mice). E qPCR analysis of Tm4sf19 expression in floating adipocytes, and MACS-separated F4/80+ and F4/80- stromal vascular fraction (SVF) isolated from GWAT of HFD-fed mice (n = 3 biologically independent replicates). F. TM4SF19 expression levels in different cell types from human white adipose tissue (GEO: GSE176171). G, H Chromatin immunoprecipitation (ChIP) enrichment analysis of recruitment of NF-ĸB to putative NF-ĸB binding site of Tm4sf19 promoter in RAW264.7 macrophages by PCR (G) and q-PCR (H). PCR amplification was carried out with input and DNA fragments immunoprecipitated by anti-NF-ĸB antibody and negative control IgG (n = 6 biologically independent replicates). I TM4SF19 and NF-ĸB expression levels of RAW264.7 macrophages after LPS treatment for 24 h (n = 6 biologically independent replicates). J Correlation analysis of TM4SF19 and NFKB1 gene expression in human subcutaneous adipose tissue and BMI (n = 12 patients). K Schematic diagram illustrating the working model of NF-ĸB-mediated transcriptional control of Tm4sf19 expression in macrophages during obesity-induced inflammation. Data are presented as mean values ± SEM. p-values were determined by the unpaired two-sided Student’s t-test (A–E, H, I), and two-tailed Pearson correlation (J). Source Data are provided as a Source Data File.

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