Fig. 4: Gene module analysis deconstructs adipose tissue macrophage subtypes.

A UMAP plot of monocyte and macrophage population from WT HFD condition. Four different colors demonstrate the different monocyte/macrophage cell types. B Tm4sf19 gene expression level (left) and module eigengenes (MEs) score of Mod4 and Mod2 (right). Tm4sf19 gene expression in the population of monocyte/macrophage from WT HFD is included in Mod4. The number of genes in each module and down- (Mod4) or up-regulated (Mod2) in the TM4SF19 KO HFD condition relative to the WT HFD condition is indicated. C Gene expression in monocyte and macrophage subtypes from WT NCD, KO NCD, WT HFD, and KO HFD condition associated with biological terms chosen from Mod4 down-regulated genes and Mod2 up-regulated genes. D Graph showing the module scores for each biological term in cells ordered along the trajectory from Trem2- to Trem2+ macrophages of GWAT of HFD-fed mice. E Expression levels of Trem2, Lyve1, Mrc1, and Itgax in macrophages. F Trajectory from Trem2- to Trem2+ macrophages on the PCA plot. The cell order along the trajectory is indicated by color. The PCs were selected to distinctly separate the Trem2+ and Trem2- macrophages. G Module scores for each biological term in the macrophage population are shown in a PCA plot. Source data are provided as a Source Data file.