Fig. 6: TM4SF19 KO restores insulin-sensitive adipocytes in adipose tissue of HFD-fed mice. | Nature Communications

Fig. 6: TM4SF19 KO restores insulin-sensitive adipocytes in adipose tissue of HFD-fed mice.

From: TM4SF19-mediated control of lysosomal activity in macrophages contributes to obesity-induced inflammation and metabolic dysfunction

Fig. 6

A, B Representative images with adipocyte size analysis (A), and adipose tissue cellularity analysis (B) of GWAT from WT and TM4SF19 KO mice fed HFD (n = 6 mice). Scale bars = 50 μm. C Schematic diagram depicting BrdU cumulative labeling. D Immunohistochemical analysis of BrdU (red) incorporation in PLIN1+ (green) adipocytes in GWAT of WT and TM4SF19 KO mice fed HFD (n = 6 mice). Scale bars = 100 μm. E tSNE plot showing the differentiation paths from APC to Adipocytes. The color indicates APC and Adipocyte clusters. The line on the tSNE plot shows the differentiation trajectory. F Heatmap of canonical marker genes used to identify each cluster. The color indicates the scaled expression level of genes. G tSNE plots showing differentiation paths split by condition. The dotted line (Adipocyte.1) highlights the cluster restored by TM4SF19 KO with HFD. H Bar plot showing the composition of APC and adipocyte clusters in each condition. I Selected pathways of GO enrichment analysis up-regulated in Adipocyte.1 or Adipocyte.3. J Violin plots showing gene expression associated with adipokine secretion, insulin sensitivity, lipolysis, and lipid accumulation. K Correlation analysis of Adipocyte.1 and Adipocyte.3 genes in human subcutaneous adipose tissue (n = 12 patients). Data are presented as mean values ± SEM. p-values were determined by the unpaired two-sided Student’s t-test (A, D, J), two-tailed Pearson correlation (K), and two-way ANOVA followed by Bonferroni post hoc tests (B). Source data are provided as a Source Data file.

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