Fig. 8: Macrophage-specific TM4SF19 KO improves obesity-induced metabolic dysfunction.
A, B qPCR (A) and immunoblot (B) analysis to confirm macrophage-specific TM4SF19 deletion from GWAT of tamoxifen-induced Tm4sf19flox/flox Csf1r-CreER (TM4SF19 MKO) mice (n = 6 mice). C, D Mouse weights monitoring (C), adipose tissue weight (D) of TM4SF19 MKO mice after HFD feeding for 12 weeks (n = 6 mice). E Body fat and lean mass of TM4SF19 MKO mice after 5 weeks or 12 weeks of HFD feeding (n = 6). F, G Glucose tolerance test (GTT) (F) and insulin tolerance test (ITT) (G) of TM4SF19 MKO mice after HFD feeding for 12 weeks (n = 6 mice). H Regression plots of energy expenditure against body mass (ANCOVA using body mass as a covariate, two-sided without adjustment, n = 6 mice). I ANCOVA -adjusted energy expenditure (EE) (predicted at the mean body mass (34.01 g at HFD 5 weeks/43.7 g at HFD 12 weeks)) (n = 6). J mRNA expression levels of genes involved in inflammatory response from GWAT of TM4SF19 MKO mice after HFD feeding for 12 weeks (n = 6 mice). K Immunofluorescence staining of F4/80 (green) with DAPI (blue) counterstaining in paraffin sections from GWAT of WT and TM4SF19 MKO HFD-fed mice. Scale bar = 100 μm (n = 6 mice). L, M Representative flow profiles of TREM2+macrophages and F4/80 expression levels (L) and BODIPY+F4/80+ macrophages and BODIPY+CD45+ cells (M) from GWAT of TM4SF19 MKO HFD-fed mice (n = 6 mice). Data are presented as mean values ± SEM. p-values were determined by the unpaired two-sided Student’s t-test (A, B, E, I–K) and two-way ANOVA followed by Bonferroni post hoc tests (D, F, G, L, M). Source data are provided as a Source Data file.
