Fig. 4: FFAR2/FFAR3 deficiency affects gene expression in IMs (inflammatory monocytes) and PMNs (pulmonary neutrophils). | Nature Communications

Fig. 4: FFAR2/FFAR3 deficiency affects gene expression in IMs (inflammatory monocytes) and PMNs (pulmonary neutrophils).

From: Clinically used broad-spectrum antibiotics compromise inflammatory monocyte-dependent antibacterial defense in the lung

Fig. 4

scRNAseq of lung cells from WT and Ffar2/Ffar3-/- mice infected with K. pneumoniae for 12 h (n = 4 for WT, n = 4 for Ffar2/Ffar3-/-). A, D, G UMAPs of AMs, PMNs, and monocytes subclusters. B, E, H Dot plots of the most differentially expressed genes for each cell subcluster. C, F, I Volcano plots of differentially expressed genes for each cell subclusters. J GSEA indicating significantly up- and downregulated pathways in IMs of infected WT and Ffar2/Ffar3-/- mice. Wilcoxon Rank Sum test was used and adjusted based on bonferroni correction using all genes in the dataset. J Bar plot indicating significantly up- and downregulated GSEA pathways in IMs of infected WT and Ffar2/Ffar3-/- mice. Calculation of p-value estimation is based on an adaptive multi-level split Monte-Carlo scheme followed by BH correction.

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