Fig. 2: Gadd45g deficiency leads to aberrations of hematological parameters and enhanced self-renewal capacities of HSCs in mice aged 4–6 months.

a Percentage of donor chimerism in the PB of lethally irradiated primary recipients transplanted with LT-HSCs freshly isolated from 4-month-old Gadd45g^+/− (GAD^+/−) (n = 4), Gadd45g^−/− (n = 4) and Ctrl (n = 5) mice together with competitor cells (top). Donor chimerism in the PB of secondary recipients transplanted with CD45.2⁺ LT-HSCs from primary recipient mice together with competitor cells (bottom). b–g Total number of BM cells (b), and absolute numbers of Gr-1⁺, Mac-1⁺ and Gr-1⁺Mac-1⁺ cells (c), B220⁺ and CD3⁺ cells (d), Ter119⁺ cells (e), CMP, GMP, MEP cells (f), and CLP cells (g) in the BM of 6-month-old Gadd45g^+/−, Gadd45g^−/− and Ctrl mice (n = 5 mice per group). h Representative images of spleens and spleen weights of 6-month-old Gadd45g^+/−, Gadd45g^−/− and Ctrl mice (n = 5 mice per group). i, j Absolute numbers of HSCs in the BM (i) and spleen (j) of 6-month-old Gadd45g^+/−, Gadd45g^−/− and Ctrl mice (n = 5 mice per group). k Numbers of My-biased HSCs and Ly-biased HSCs in the BM from 6-month-old Gadd45g^+/−, Gadd45g^−/− and Ctrl mice (n = 5 mice per group), and from the moribund mice with MPN (n = 25) and Ctrl (n = 10) group (bottom). Representative flow cytometry plots were shown on the top. l Absolute numbers of MPP2, MPP3 and MPP4 in the BM of 6-month-old Gadd45g^+/−, Gadd45g^−/− and Ctrl mice (n = 5 mice per group). m–o Apoptosis (m), proliferation (n) and cell-cycle (o) analysis of My-biased HSCs in the BM from 6-month-old Gadd45g^+/−, Gadd45g^−/− and Ctrl mice (n = 4 mice per group). p Percentages of donor-derived overall (CD45.2⁺) cells in the PB of primary recipients transplanted with My-biased HSCs from 6-month-old Gadd45g^+/− (n = 4), Gadd45g^−/− (n = 4) and Ctrl (n = 5) mice together with competitor cells (top), and chimerism in the PB of secondary recipients transplanted with CD45.2⁺ My-biased HSCs from primary recipients together with competitor cells (bottom). q Percentages of donor chimerism in the PB of primary recipients transplanted with My-biased HSCs from 8-month-old Gadd45g^+/−, Gadd45g^−/− and Ctrl mice together with competitor cells (left), and in the PB of secondary recipients transplanted with CD45.2⁺ My-biased HSCs from primary recipients together with competitor cells (right). (n = 5 mice per group). For (a–q): Data are shown as means ± SD, two-tailed Student’s t-test.