Fig. 5: Validation dataset.
From: Nonlinear DNA methylation trajectories in aging male mice
a Overlap between differentially methylated regions in aging (aDMR) CpGs in the original and validation datasets. b–f Distribution of mean methylation levels by age group in the original dataset (left) and in the validation dataset (right) and the methylation Z-score trajectories during aging for clusters C1–C5, respectively. The yellow line indicates the cubic smooth spline of the Z-scores in the original dataset. For the validation dataset, 70% of the cytosines closest to the original trajectory in each cluster are plotted. Number of CpGs: n = 36,243 (C1 original) n = 24,920 (C1 validation); n = 4345 (C2 original) n = 2956 (C2 validation); n = 2989 (C3 original) n = 2025 (C3 validation); n = 5091 (C4 original) n = 3502 (C4 validation); n = 882 (C5 original) n = 602 (C5 validation). For the box plots, the center line shows the median, the box limits show the first and third quartiles, and the upper and lower whiskers extend from the hinge to the largest or the lowest value no further than 1.5× the interquartile range (IQR) from the hinge. Source data are provided as a Source Data file.
