Fig. 1: Single-cell atlas of adult Pristina leidyi annelids.
From: Annelid adult cell type diversity and their pluripotent cellular origins
A Experimental workflow. Cartoon adapted from reference. 44. B UMAP visualisation of the 75,218-cell Pristina leidyi single-cell transcriptomic atlas with clusters coloured according to their cell identity. C Expression plots of markers of the major broad types, including epidermis, gut, muscle, neurons, globin+ cells, polycystin cells, eleocytes, chaetal sacs and lipoxygenase+ cells. D Lineage reconstruction abstracted graph (PAGA) showing the most probable path connecting the clusters. Each node corresponds to a cell cluster identified with the leiden algorithm. The size of nodes is proportional to the amount of cells in the cluster, and the thickness of the edges is proportional to the connectivity probabilities. Nodes are coloured according to their cell identity. E Cell cluster percentages at the broad cell type and the cell type levels.
