Fig. 2: Functional identification of the 2-CGTs and 4-OMTs involved in the biosynthesis of bergenin.
From: Unravelling and reconstructing the biosynthetic pathway of bergenin
a The organs (leaf, stem, and rhizome) of A. japonica were used to analyze the contents of bergenin (1) and transcriptome sequencing. b The contents of bergenin (1) in different organs, all data represent the means of three parallel experiments and error bars show standard deviation. c Expression heat-map of two candidate CGT genes and ten OMT candidate genes (AjOMT1−10) in different organs. d–f The reactions catalyzed by AjCGT1 and AjCGT2 with GA (2) and UDP-Glc as substrates, and the corresponding HPLC-MS/MS2 spectra. g–i The reactions catalyzed by AjCGT1 and AjCGT2 with 4-OMGA (3) and UDP-Glc as substrates, and the corresponding HPLC-MS/MS2 spectra. j–l The reactions catalyzed by AjOMT2 with GA (2) and SAM as substrates, and the corresponding HPLC-MS spectra. m–o The reactions catalyzed by AjOMT2 with norbergenin (6) and SAM as substrates, and the corresponding HPLC-MS spectra. Source data are provided as a Source data file.
