Fig. 5: Boosting the SAM and UDP-Glc supplies to improve the yield of 4-OMGA-Glc de novo.
From: Unravelling and reconstructing the biosynthetic pathway of bergenin
a Modular biosynthetic pathway of 4-OMGA-Glc (4) in engineered E. coli. b Biosynthetic pathways of UDP-Glc and SAM as well as the SAM regeneration system in E. coli. c Reconstruction of engineered strains D1–D5 in E. coli BL21. d Evaluation of the engineered strains D1–D5 for titer of 4-OMGA-Glc (4) and metabolic intermediates in shake flask fermentation for 48 h. AjCGT1opt, codon-optimized AjCGT1; AjOMT2*opt, codon-optimized AjOMT2-Y203T mutation. S-adenosyl-L-homocysteine (SAH) can be catalyzed by 5′-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtn) to produce S-ribosylhomocysteine (SRH), which can be further converted to homocysteine (Hcys) by S-ribosylhomocysteine lyase (luxS). Hcys can be further transformed to methionine (Met), which is finally catalyzed by methionine adenosyltransferase (metK) to form SAM. Phosphoglucomutase (pgm) catalyzes glucose-6-phosphate to form glucose-1-phosphate which is catalyzed by glucose−1-phosphate uridyltransferase (galU) to form UDP-Glc. All data represent the means of three parallel experiments and error bars show standard deviation. Statistical analysis was performed by using the Student′s t test. P value for each comparison from left to right in d): 0.0010 (**), 0.0024 (**), 0.0065 (**), 0.0014 (**), 0.0448 (*), 0.0094 (**), 0.0045 (**), 0.0143 (*), 0.0058 (**). Source data are provided as a Source data file.
