Fig. 2: In vivo and in vitro characterization of TgCatJp5.

a Evaluation of in vivo virulence in mice. The pathogenicity of TgCatJp5 in mice was evaluated by inoculating 1000 tachyzoites in C57BL/6 (n = 5, each) or Ifngr1^−/− mice (n = 5) in parallel with RH and ME49. All mice inoculated with RH died by day 9 after inoculation, while mice inoculated with ME49 and TgCatJp5 all survived to day 30 post-infection. Ifngr1^−/− mice succumbed to infection completely by day 11 post-TgCatJp5 infection. Source data are provided as a Source Data file. b Accumulation of Irgb6 on PV membranes. Accumulation of Irgb6 into PV membrane was tested by infecting IFN-γ-stimulated mouse embryonic fibroblasts (MEFs) with the parasites HG1 (RH), HG2 (ME49), and TgCatJp5. Two hours later, cells were fixed, and immunofluorescence staining was performed using GRA7 (green), Irgb6 (red), and DAPI (blue). The scale bar represents 10 μm. N = 3 biologically independent samples, n = 100 cells were examined over experiment. Arrowheads indicate Irgb6+ parasitophorous vacuoles. Source data are provided as a Source Data file. c Copy number variation of ROP5. Sequencing depth at every 1000 bp was calculated by tinycov. CNV was calculated by dividing the maximum sequencing depth of the ROP5 gene region by the average sequencing depth of chromosome XII. Dashed lines represent estimated copy depth of the archetype strains (blue, type I/RH-88; green, type II/ME49; red, type III/VEG). d Mapping status for the UPS region of ROP18. Sequencing depth was calculated by tinycov every 50 bp, showing the 1,515,500–1,516,500 bp range of chromosome VIIa containing the UPS region of ROP18. e Relationship between TgCatJp5 and TgCatJpOk4 assumed from the allele exchange of ROP5 and ROP18.