Fig. 8: IL-4 and IL-3 control PD-L1 expression, activation and localization of basophils in lupus-like models.

a PD-L1 expression levels on basophils from spleen (left) (n = 22/14/5/8) and lymph nodes (LN) (right) (n = 13/10/6/8) from Mcpt8CT/+ (WT) (blue) or Mcpt8CT/+Il4fl/fl (Il4fl/fl) (red) mice injected with PBS (–) or with pristane (+). b IL-3 titers (in pg/mL) in the plasma from Mcpt8CT/+ (WT) (blue), Mcpt8CT/+Il4fl/fl (Il4fl/fl) (red), Mcpt8CT/+Pdl1fl/fl (Pdl1fl/fl) (green), basophil-deficient (Mcpt8CT/+R26DTA/+) (gray) mice injected with PBS (–) or with pristane (+) and in aged (min. 30 weeks old) Mcpt8DTR (WT) and Lyn–/–Mcpt8DTR mice (pink) (n = 7/12/6/8/4/5/6/5/3/3). c Aged Lyn–/– mice were injected with isotype control (rat IgG1,κ; Iso; black), rat anti-mouse IL-3 (αIL-3; blue) or rat anti-mouse IL-4 (αIL-4; red) as indicated. PD-L1 (d) (n = 7/9/6) and CD200R1 (e) (n = 6/9/6) expression levels on basophils from spleen of mice described in (c) (Left) as measured by flow cytometry (Right). f Proportions (%)of basophils among CD45+ splenocytes of mice as in (c) (n = 7/9/6). g Proportions (%) of TFH cells among CD4+ T cells from spleen of mice as in (c) (n = 4/6/6). h Proportions of CD19+CD138+ cells among CD45+ cells from lymph nodes (LN) (%) of mice as in (c) (n = 4/6/6) (Right) as measured by flow cytometry (Left). a, b, d–h Results are from at least two independent experiments and presented as individual values in bars representing the mean values ± s.e.m. a, b, d–h Statistical analyses were done by two-way ANOVA test followed by Tukey’s multiple comparisons tests (a), by one-way ANOVA test followed by two-sided unpaired t tests (b) between the indicated groups, or by one-way ANOVA test followed by Tukey’s multiple comparisons tests (d–h) between the indicated groups. P values are shown above each bracket. NS not significant. Source data are provided in the Source Data file.