Fig. 2: The expression of LKRSDH in both neurons and glial cells is necessary for maintaining the circadian rhythm in aged flies.
a–o LKRSDH represented by GFP in LKRSDHMB01942 was expressed in the same set of cells with neurons labeled with Elav antibody or glial cells labeled with Repo antibody. Heads of young LKRSDHMB01942 were dissected at ZT8. The arrow highlights the co-localization of LKRSDH with neurons or glial cells. Cells surrounded by rectangle and circle shapes represent the co-localization of LKRSDH with glial cells and neurons, respectively. Scale bar = 10 µm. The images presented are from one of the three biological repeats. p Percentage of rhythmic and arrhythmic flies (from left to right, bars represent n = 30, 31, 32, 31, 28, 30, 28, 28, 29, 31, 44, 21, 31, 24, 31, 41, 22, 26, 28, 26, 27 and 27 independent samples). P-values from two-sided Fisher’s exact test are indicated, where *P < 0.05, ***P < 0.001 and ****P < 0.0001. All the P-values are listed in Supplementary Data 3. q Percentage of rhythmic and arrhythmic flies (from left to right, bars represent n = 36, 27, 42, 38, 32, 26, 30, 30, 34 and 41 independent samples). P-values from two-sided Fisher’s exact test are indicated, where ****P < 0.0001. All the P-values are listed in Supplementary Data 3. r Percentage of rhythmic and arrhythmic flies (from left to right, bars represent n = 32, 27, 42, 36, 37, 30, 30, 30, 42 and 34 independent samples). LKRSDHMB01942,UAS-dCas9/+, LKRSDHMB01942,UAS-dCas9/LKRSDHOE;repo-Gal4/+ and LKRSDHMB01942,UAS-dCas9/LKRSDHOE;nsyb-Gal4/+ are LKRSDH heterozygotes mutant flies. P-values from two-sided Fisher’s exact test are indicated, where ****P < 0.0001. All the P-values are listed in Supplementary Data 3. Source data are provided as a Source Data file.
