Fig. 4: Overexpression or knockout of dilps resulted in circadian rhythm defects.

a Quantitative RT-PCR of dilp2, dilp3, dilp5, LKRSDH and art4. Data are presented as mean ± SEM. P-values from unpaired two-tailed Student’s t-test are indicated, where n.s. indicates no significant difference, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. All the P-values are listed in Supplementary Data 3. n = 3 biologically independent experiments. b Percentage of rhythmic and arrhythmic flies (from left to right, bars represent n = 32, 32, 32, 31, 31, 30, 27, 24, 28 and 29 independent samples). P-values from two-sided Fisher’s exact test are indicated, where *P < 0.05 and ****P < 0.0001. All the P-values are listed in Supplementary Data 3. c Percentage of rhythmic and arrhythmic flies (from left to right, bars represent n = 31, 37, 26, 26, 38 and 28 independent samples). LKRSDH^MB01942/+ and LKRSDH^MB01942/+; dilp1-5^KO/+ are LKRSDH heterozygotes mutant flies. P-values from two-sided Fisher’s exact test are indicated, where ***P < 0.001. All the P-values are listed in Supplementary Data 3. d Percentage of rhythmic and arrhythmic flies (from left to right, bars represent n = 28, 27, 32, 37, 32, 27, 46, 33, 58, 26, 31, 30, 33, 27, 30, 28, 29 and 32 independent samples). LKRSDH^MB01942/ + , LKRSDH^MB01942/+; dilp2¹/+, LKRSDH^MB01942/+; dilp3¹/+, LKRSDH^MB01942/+; dilp5¹/+, LKRSDH^MB01942/+; dilp3¹/dilp5¹ are LKRSDH heterozygotes mutant flies. P-values from two-sided Fisher’s exact test are indicated, where *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. All the P-values are listed in Supplementary Data 3. Source data are provided as a Source Data file.