Fig. 5: Native MS and mass photometry analysis of LAT1-4F2hc super-dimerization.

A Simulation of possible N-glycan conformers on LAT1-4F2hc. N-glycan conformers are highlighted in dark purple. B Structural illustration of the modelled structure of LAT1-4F2hc super-dimer with possible N-glycan conformers. The 4F2hc-Asn365 and Asn424 are proximal to the super-dimer interface. The N-glycans fill the interface and mediate hydrophilic interactions between two 4F2hc subunits in the LAT1-4F2hc super-dimer. C Native mass spectra of LAT1-4F2hc WT, desialylated WT and non-glycosylated M4 mutant. The LAT1-4F2hc heterodimer and its super-dimer peaks are highlighted (blue and red, respectively). D Mass photometry analysis of LAT1-4F2hc fully sialylated WT (control), desialylated WT and sialylated WT at pH 5.0. E Bargraphs show the super-dimer/heterodimer ratios of fully sialylated WT (control), desialylated WT and sialylated WT at pH 5.0. Bars show mean ± standard deviation from three independent experiments (dots). Two tailed Student’s t-tests are performed for statistical analysis (p < 0.01 was labelled with two asterisks). Source data are provided as a Source Data file. F Surface electrostatic potential of the super-dimer interface of 4F2hc subunit at pH 7.0 and pH 5.0.