Fig. 6: Probing the endogenous LAT1-4F2hc assemblies. | Nature Communications

Fig. 6: Probing the endogenous LAT1-4F2hc assemblies.

From: The complete assembly of human LAT1-4F2hc complex provides insights into its regulation, function and localisation

Fig. 6

A Western blotting analysis of the in vivo crosslinked (XL) endogenous LAT1-4F2hc in HeLa cells. An anti-LAT1 antibody was used to probe the endogenous LAT1-4F2hc assemblies without BS3 (-) and with BS3 (+) treatments. The experiment was repeated once. Source data are provided as a Source Data file. B Correlation of the mRNA expression levels of 4F2hc (SLC3A2) and its associated members of the SLC7A family (SLC7A5, SLC7A6, SLC7A7, SLC7A8, SLC7A9, SLC7A10) from human tissue and cell lines. The mRNA expression levels (measured in normalized transcripts per million, nTPM) of 4F2hc and SLC7As from 50 human tissues and 1205 cell lines were plotted as a scatter plot. The linear-fit trendline is plotted as a dark blue line. The shaded areas corresponding to one and two standard deviations (σ) of the linear regression are highlighted (dark blue and light blue, respectively). Source data are provided as a Source Data file. C Expression levels of SLC3A2 and SLC7A5/6/7/8/10/11 in HeLa cell lines. Source data are provided as a Source Data file. D Western blotting of the endogenous LAT1-4F2hc assemblies in HeLa cells without and with dithiothreitol (DTT) treatment. The experiments were repeated once. Source data are provided as a Source Data file. E Model of the dynamic assemblies of LAT1-4F2hc super-dimer with highlighted key findings. The LAT1 homo-dimer and LAT1-4F2hc super-dimer structures are proposed based on AlphaFold-Multimer and homology-modelling.

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