Fig. 2: Hmg1-mediated triazole resistance is uniquely driven by mutations in the predicted sterol sensing ___domain.
From: A secondary mechanism of action for triazole antifungals in Aspergillus fumigatus mediated by hmg1
A Schematic of the protein ___domain structure of A. fumigatus Hmg1. The ___location of mutations identified in genome sequences selected for this study are indicated. The previously characterized S305P mutation is indicated for context. Illustration prepared using BioRender.com. Susceptibility profiles of the parental control strain (ΔakuB-pyrG+), the manipulation control strain (hmg1WT), and the indicated hmg1 mutants to mold-active triazoles are shown for intraconazole (B), posaconazole (C), voriconazole (D), and isavuconazole (E). CLSI-based minimum inhibitory concentration (MIC) assays were performed in triplicate for each strain. Red font indicates mutant strains with shifts in susceptibility compared to controls.
