Fig. 2: Cryo-EM structure of Na_VEh^ΔN.

a The cryo-EM map of Na_VEh^ΔN. The four subunits and lipids are colored in green, light purple, yellow, red, and gray, respectively. The same color codes are applied for Na_VEh^ΔN unless specified. b Cartoon representation of Na_VEh^ΔN. c The activation gate of Na_VEh^ΔN (upper panel) and Na_VEh^WT (lower panel). The Na_VEh^WT map is colored in gray, and the N-helix is highlighted in cyan. d, e Ion path of Na_VEh^ΔN (d) and Na_VEh^WT (e) calculated by HOLE. Residues at the constriction sites of selectivity filter are shown side chains in sticks. The red dashed boxes indicate the regions of intracellular activation gates. f Pore radii of Na_VEh^ΔN and Na_VEh^WT from d, e. The pore radii of Na_VEh^ΔN and Na_VEh^WT are colored cyan and light purple, respectively.