Fig. 7: Injection of ferritin partially rescues Evi5-impaired larvae.

A Survival analysis of PG>Fer1HCH^IR animals injected with ferritin solutions. Commercially obtained apo- and holo-ferritin isolated from equine spleen were injected into of PG>Fer1HCH^IR second instar larvae and scored for surviving animals at larval, pupal and adult stages. L2: 2^nd instar, L3: 3^rd instar, P: pupae, Ad: adults. B Charts depicting the elapsed time for control (PG > w¹¹¹⁸) and PG >FLP;Evi5^FRT embryos to reach the prepupal stage. Denatured holo-ferritin served as a negative control. In (A, B) error bars represent standard error of three biological replicates (each = 50 individuals), with the center indicating the average. C PG cells protoporphyrin accumulation analysis of PG >FLP;Evi5^FRT larvae injected with indicated compounds under UV light. BF: bright field. Scale bars = 250 μm. D Expression analysis of the ALAS in ring gland samples isolated from injected PG >FLP;Evi5^FRT larvae. Fold changes calculated with ddCT method and error bars represent 95% confidence intervals, both are based on three biological replicates (each tested in triplicate). The center of error bars denotes the average. Asterisks denote P-values calculated with the two-sided Student’s t-test (***p < 0.001). Source data are provided in the source data file.