Fig. 1: The PATTERN concept. | Nature Communications

Fig. 1: The PATTERN concept.

From: Photoacoustic Tomography with Temporal Encoding Reconstruction (PATTERN) for cross-modal individual analysis of the whole brain

Fig. 1

a The pipeline for PATTERN imaging. Partially created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en. b Schematic of the PATTERN system. The agar-based sample holder enables intact brain imaging without the need for embedding, and the inlet and outlet were perfused with phosphate buffer solution (PBS) or 4 °C artificial cerebrospinal fluid (aCSF) to maintain biological activity. Inset: the FOV of PATTERN is a cylinder with a diameter defined by the translation distance. c Imaging sequence of PATTERN. A total of 32 rotations and 1024 translation steps were performed in a single scan cycle. A typical bleaching process includes 8 scan cycles, taking approximately 17.5 mins. d–g PATTERN images of a mouse brain whose hippocampus was labeled with iRFP713 and EGFP. d Bottom view of a 3D-rendered volume. Insets: magnified views of a projection feature. e–g Tomographic views from three orthogonal perspectives related to planes in (d). The bleaching curve of the point indicated by the white arrow is shown in Fig. 2e.

Back to article page