Fig. 5: Combination of PATTERN imaging with fMOST or LSFM. | Nature Communications

Fig. 5: Combination of PATTERN imaging with fMOST or LSFM.

From: Photoacoustic Tomography with Temporal Encoding Reconstruction (PATTERN) for cross-modal individual analysis of the whole brain

Fig. 5

a Schematic of the cross-modal correction strategy. Partially created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en. b–f Cross-modal correction applied to fMOST and LSFM images (b), 3D and representative coronal sections of the brain that underwent fMOST; (d), 3D and representative coronal sections of the brain that underwent uDISCO; (c) and (e), Comparison of coronal sections before and after correction. f Horizontal sections of an AD rat brain that underwent iDISCO. The yellow dashed line represents the contour of fMOST or LSFM, white represents PATTERN, and blue represents the corrected result. g Schematic of AAV expressing EGFP and iRFP713 fusion protein. h Experimental flowchart for correction of fluorescence signals via PATTERN. Partially created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en. i Representative coronal sections of brain with fusion protein that underwent PEGASOS. j 3D structure of the brain with fusion protein. k–m Magnified view of the regions indicated by the colored box in (j). White arrows represent the false-positive signals; yellow represents the unbleachable signal, dSub dorsal subiculum, EC entorhinal cortex, MM medial mammillary nucleus, RSP retrosplenial area, NDB nucleus of the diagonal band, OB olfactory blub, HY hypothalamus.

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