Fig. 7: Biocompatibility assessment of R-SEA-Si. | Nature Communications

Fig. 7: Biocompatibility assessment of R-SEA-Si.

From: Molecular orbital breaking in photo-mediated organosilicon Schiff base ferroelectric crystals

Fig. 7

a Schematic of in vitro biocompatibility assessment of R-SEA-Si towards MC3T3-E1 cells. b Cell viability of MC3T3-E1 cells after incubation with leach liquor of R-SEA-Si for 24 and 48 h respectively. The data was presented as mean values +/– SD. P < 0.05 was considered statistically significant, which was marked with the * symbol. c Representative scan of live/dead staining of MC3T3-E1 cells after treated with leach liquor of R-SEA-Si for 24 and 48 h, green and red labeled cells represent living and dead cells, respectively. d Quantification of live/dead staining in Fig. 7c using ImageJ. The data was presented as mean values +/– SD. e Representative scan of phalloidine/DAPI staining of MC3T3-E1 cells after treated with leach liquor of R-SEA-Si for 24 and 48 h, green and blue labeled cells represent cytoskeleton and nucleus of cells, respectively. 3 independent biological replicates were conducted. f Schematic of in vivo biosafety assessment of R-SEA-Si by subcutaneously implantation of R-SEA-Si (10 mg) into ICR mice. g Serum biochemical test results from control and R-SEA-Si administered mice at 3 days after subcutaneously implantation (n = 5 per group). The data was presented as mean values +/- SD. h H&E staining images of mouse skin tissue directly above the implanted R-SEA-Si. 3 independent biological replicates were conducted. i H&E staining images of major organs collected from control and R-SEA-Si administered mice at 3 days after subcutaneously implantation. 3 independent biological replicates were conducted. Figure 7(a) and (f), created with BioRender.com, released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.

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