Fig. 4: Spatial and spectral characteristics of the MCoSM image of the BPAE.
From: Mechanical-scan-free multicolor super-resolution imaging with diffractive spot array illumination
The combination of reconstructed MCoSM images obtained at wavelengths of 461 nm, 512 nm, and 599 nm, showing the distribution of the nucleus (blue), cytoskeleton (green), and mitochondria (red) over the FoV. a, b MCoSM images of organelles obtained by 10 × 10 and 50 × 50 super-resolution spot arrays with resolutions of 0.52 Airy and 0.68 Airy, respectively. c Widefield image of organelles obtained with the same objective (objective lens: 60×/0.90 NA). The region of interest (ROI) marked by the white solid rectangle is shown in the enlarged upper right box. Comparisons of identical areas are marked with white dotted rectangles. d Intensity profiles of the cytoskeleton on the selected line in (a) (SR1), (b) (SR2), and (c). The effective FoV and spatial resolution of the white dotted box regions in Fig. 3b, c were compared with those of the region in Fig. 3a. Scale bar: (a) 5 μm, (b) and (c) 25 μm (see Supplementary Movies 2 and 3).
