Fig. 4: Trial-to-trial registration in vivo.
From: Magnetic voluntary head-fixation in transgenic rats enables lifespan imaging of hippocampal neurons
A Histogram of the displacement of the brain in the z-axis, axial to the focal plane, for two sessions in two rats. Inset shows the calculated frame-by-frame z-axis displacement for a single trial with a large range displacement. B Shows the average fluorescence fields of view for two 0.5 s periods at either end of the z-axis displacement range for the single trial inset in A. At the extreme range of such displacements, the same fine structures such as dendrites are visible in the image. C Histogram of the between trial registration displacements for six rats. Inset shows the spread of trial-to-trial displacements for a single session. D Histogram of the within-trial brain motion in x (anterior–posterior) and y (medio–lateral) for all rats. Inset shows the time course of the x and y displacements for a single trial.
