Fig. 3: In vitro neutralization of toxin activity by VHHs. | Nature Communications

Fig. 3: In vitro neutralization of toxin activity by VHHs.

From: In vivo neutralization of coral snake venoms with an oligoclonal nanobody mixture in a murine challenge model

Fig. 3

A Inhibition of PLA2 enzymatic activity by VHHs. The maximal enzyme activity observed with toxin alone was set to 100%. The normalized enzymatic activity of PLA2s from various elapid genera (PLA2N, Nn19, and Hh3) preincubated for 30 min at RT with anti-PLA2 VHHs at a 1:20 toxin to VHH molar ratio. Bars represent the mean of two replicates. B Neutralization of αNTx-mediated blocking of muscle-type nAChR current. Dose-response curves from patch clamp experiments with increasing concentrations of VHHs to prevent the blocking of nAChR by 15 nM αNTx DH or 5 nM scNTx. Error bars represent standard deviation of independent cells performed in a 384-well plate. n = 16. Source data are provided as a Source Data file.

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