Fig. 5: PhoB regulates the expression of sodB. | Nature Communications

Fig. 5: PhoB regulates the expression of sodB.

From: Phosphorus deficiency alleviates iron limitation in Synechocystis cyanobacteria through direct PhoB-mediated gene regulation

Fig. 5

a Verification of the interaction between the cis-acting element pro-sodB and the transcription factor PhoB by Y1H assay. b Interaction analysis between pro-sodB and PhoB by EMSA. The concentration of PhoB added into each mixture is shown. Unlabeled DNA (SC) and poly (dI-dC) (NSC) were added to examine the specificity of the binding reactions. Similar results were obtained from three independent experiments. c The relative transcript levels of sodB in the WT strain and phoB mutant after 4 days of growth under different Fe and P availabilities. Values are mean ± SD of three independent biological replicates, and the results were normalized to the transcript levels measured under +Fe+P conditions. d Intracellular ROS content of WT and the phoB mutant after 4 days of growth under +Fe+P, +Fe−P, −Fe+P, and −Fe−P conditions. Data shown are mean ± SD of three independent biological replicates, and the results were normalized to the ROS level of +Fe+P treatment. e Representative images of each strain grown in the absence and presence of 0.3 µM MV. The experiment was repeated with similar results three times. For c and d letters above bars indicate statistical significance (P < 0.05) calculated by one-way ANOVA across all treatments. Source data are provided as a Source Data file.

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