Fig. 1: The H2AX-(X)pSQEY is the minimal DDR activating motif.
From: Evolved histone tail regulates 53BP1 recruitment at damaged chromatin
a Sequence alignment of human H2A and H2AX. Conserved residues are highlighted in black. b Sequence alignment of H2AX homologs C-terminal tail sequence across different species. Conserved residues are highlighted in black. c Schematic illustration of the H2AX-mediated phosphorylation-ubiquitination DNA damage response pathway. d Immunoblots of H2AX and γH2AX antibodies for H2AX mutants. Repeated three times independently with similar results. e Representative immunofluorescence micrographs of ionizing radiation induced foci for γH2AX, MDC1 and 53BP1 in H2AX KO with GFP-H2AX deletion mutant reconstitution at 1 h after 10 Gy radiation. f Quantification of 53BP1 foci as represented in (e) for the indicated H2AX mutants. The error bars correspond to mean ± SD of three independent experiments. Two-tailed unpaired T test. Source data are provided as Source Data file.
