Fig. 6: The H2AX C-terminal linker region-mediated 53BP1 IRIF formation is cell cycle regulated.
From: Evolved histone tail regulates 53BP1 recruitment at damaged chromatin
a Quantification of the total number of foci per cell in H2AX KO cells expressing H2AX wildtype or Y142L 1 h after 10 Gy radiation. Data represents ≥25 cells from three independent experiments and the line is representative of the mean. Two-tailed unpaired T test. b Representative immunofluorescence images for 53BP1 in H2AX KO cells with GFP-H2AX wildtype or mutant reconstitution, 1 h after 1 µM camptothecin treatment. c Quantification of cells with >5 53BP1 foci in H2AX KO cells expressing GFP-H2AX and mutants as shown in (b). The error bars correspond to mean ± SD of three independent experiments. Two-tailed unpaired T test. d Quantification of the total number of 53BP1 foci per cell in H2AX KO cells with reconstitution of GFP-H2AX wildtype or mutant reconstitution 1 h after 1 µM camptothecin treatment as shown in (b). Data represents ≥25 cells from three independent experiments and the line is representative of the mean. Two-tailed unpaired T test. e Representative images of clonogenic survival assay of H2AX KO cells with or without stable expression of SFB-H2AX wildtype or Y142L and treated with indicated doses of camptothecin. f Quantification of cell survival as represented in (e). The error bars correspond to mean ± SD of three independent experiments. Source data are provided as Source Data file.
