Fig. 1: Purification and cryo-EM structure of the yeast CORVET complex. | Nature Communications

Fig. 1: Purification and cryo-EM structure of the yeast CORVET complex.

From: Structure of the endosomal CORVET tethering complex

Fig. 1

A Size exclusion chromatography (SEC) of the affinity-purified CORVET. The representative result of three independent experiments is shown. B SDS-PAGE analysis of purified CORVET. Protein samples from affinity purification (eluate) and before and after SEC are shown. n = 3 independent experiments were performed. C Mass photometry analysis of the peak fractions from SEC of CORVET. D Overall architecture of the CORVET complex. Composite cryo-EM map generated from local refinement maps (see Figs. S2, S3) is colored by subunits assigned (Vps3, violet; Vps8, pink; Vps11, light blue; Vps16, sand; Vps18 blue; Vps33, brown). The consensus maps used for local refinements are low-pass-filtered and shown as a transparent envelope with a black outline. E Molecular model of CORVET fitted into the low-pass-filtered consensus map.

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