Fig. 5: Collagen I induced forces control Yap-mediated transcription and invasion.
From: A YAP-centered mechanotransduction loop drives collective breast cancer cell invasion
a Representative bright-field images of MMTV-PyMT organoids embedded in attached (Att.) and floating (Flo.) Collagen I gels. Arrowheads: invasive strands. b Percentage of organoids that have 1 or more invasive strands (invasive organoids). Data show average values with SD from n = 110 (Att.) and n = 165 (Flo.) organoids from five independent experiments. P values, two-sided unpaired Mann–Whitney test. c qPCR data showing relative mRNA expression of the classical Yap targets and other genes (identified from single cell sequencing) in MMTV-PyMT organoids embedded in floating relative to attached Collagen I gels. The bar graph represents the average values with SD from 3 independent experiments. P values, two-sided Student’s t test. d Yap distribution in rim and leader cells after detaching the Collagen gels for 2 h 30 min. e Yap nuclear/cytoplasmic ratio in the K14 positive cells that are either located at the rim or at leader positions in attached and floating gels. Data are derived from 29 to 40 cells per condition from three independent experiments. P values, two-sided Kruskal–Wallis test with Dunn’s multiple comparison. Scale bars: 50 μm (a, d), 10 μm (d, inset). Source data are provided as a Source Data file.
