Fig. 2: The dynamics of microtubules and actin filaments in pollen. | Nature Communications

Fig. 2: The dynamics of microtubules and actin filaments in pollen.

From: Distinct functions of microtubules and actin filaments in the transportation of the male germ unit in pollen

Fig. 2

a Time-lapse images of mCherry-MBD-labeled microtubules and Lifeact-mEGFP-labeled actin filaments shortly after hydration in pollen grains. “0 s”, the point when mature pollen was unhydrated. Bar, 5 μm. b Fluorescence intensity of mCherry-MBD and Lifeact-mEGFP along the white lines in (a). c Time-lapse images of Lifeact-mEGFP-labeled actin filaments at cell cortex treated by LatB. Bar, 2 μm. d Time-lapse images showing the arrangement and dynamics of microtubules and actin filaments in the germinating pollen grains. Bar, 5 μm. e Relative fluorescent density of mCherry-MBD and Lifeact-mEGFP at Sites 1 and 2 in (d). f Kymograph analysis of mCherry-MBD and Lifeact-mEGFP in the region indicated by the white band in (d). The region marked with yellow dotted squares is longitudinally drawn. g Representative images of 20 images showing mCherry-MBD and Lifeact-mEGFP in pollen tubes. Bar, 10 μm. h Cross-section images of mCherry-MBD and Lifeact-mEGFP at the subapical region of the pollen tube in (g). Bars, 2 μm. i Fluorescence intensity of mCherry-MBD and Lifeact-mEGFP at the subapical region of the pollen tube along the white line in (h). j Cross-section images of mCherry-MBD and Lifeact-mEGFP at the apical region of the pollen tube in (g). Bars, 2 μm. k Fluorescence intensity of mCherry-MBD and Lifeact-mEGFP at the apical region of the pollen tube along the white line in (j). Source data are provided as a Source Data file.

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