Fig. 4: The stable directional movement of VN in pollen relies on microtubules. | Nature Communications

Fig. 4: The stable directional movement of VN in pollen relies on microtubules.

From: Distinct functions of microtubules and actin filaments in the transportation of the male germ unit in pollen

Fig. 4

a Time-lapse images of mCherry-MBD and H2B-GFP in germinating pollen grains upon the application of oryzalin. “0 s”, 10 min after the incubation of pollen grains on a medium containing 1.5 μM oryzalin. Bar, 5 μm. b Trajectory analysis of VN movement in (a). Bar, 5 μm. c Quantification of the maximum distance of VN movement in pollen grains without (n = 25) or with (n = 26) oryzalin treatment. d, e Time-lapse images of VN (green, arrows) and SCs (magenta) in DMSO (d) or oryzalin-treated growing pollen tubes (e). Bar, 30 μm. f, g Kymograph analysis of (d) and (e). The pollen tube tip is indicated with an arrow. h Quantification of the velocity of VN movement in DMSO and oryzalin-treated pollen tubes. Velocity of VN forward movement, DMSO (n = 494), Oryzalin (n = 270); velocity of VN backward movement, DMSO (n = 148), Oryzalin (n = 229), n from 10 cells for each treatment. i The frequency distribution of the distance of VN to the tip of DMSO and oryzalin-treated pollen tubes. j Quantification of the minimum and maximum distance of VN to the tip of DMSO and oryzalin-treated pollen tubes. n = 10 pollen tubes for each treatment. k Time-lapse images of mCherry-MBD and H2B-GFP in pollen tubes after oryzalin washout. The region marked with white dotted squares is enlarged in the right panels. The reassembly sites of microtubules are indicated by arrows, and the contact sites of VN tips and microtubules are pointed out with arrowheads. n = 5. Bar in the left panel, 10 μm; bar in the right panel, 5 μm. l Kymograph analysis of (k). Values are mean ± SD; * P < 0.05,*** P < 0.001; two-tailed Student’s t-test in (c), (h) and (j). Source data are provided as a Source Data file.

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