Fig. 1: METTL3 downregulation restores the physiological RNA composition of stress granules in ALS cellular models.

a Schematic representation of the experimental flow used for comparing stress granule transcriptomes. b–d Scatter plot depicting RNA differential enrichment in SG in FUSWT vs FUSWT METTL3-KD (b) or FUSP525L vs FUSP525L METTL3-KD (c), or FUSWT vs FUSP525L METTL3-KD (d) conditions. Axes describe log2FC of SG RNA enrichment in the indicated conditions. Red dots indicate ΔMETTL3-enriched RNAs. Blue dots indicate ΔMETTL3-depleted RNAs. Black dots indicate commonly enriched RNAs. Gray dots indicate invariant RNAs. n = 2 biologically independent replicates. e Pie charts displaying the percentage of meRIP-Seq enriched RNAs among invariant, commonly enriched, and relocated defined in the comparison between FUSP525L vs FUSP525L METTL3-KD. n = 2 biologically independent replicates. Source data are provided as a Source Data file.