Fig. 4: METTL3 chemical inhibition relieves FUS-containing SG.
a Representative images of the indicated stressed patients-derived fibroblasts. G3BP1 antibody staining is depicted in green and FUS in red. The nuclei are stained with DAPI (blue). The merge of the signal is shown. The scale bar is 10 µm. (n = 3 biologically independent replicates.) b, c Box plots illustrating the number of stress granules per cell in stressed iPS-derived MN expressing FUSP525L or patient-derived fibroblasts treated either with DMSO or STM-2457. The box plots are defined by minima, 25% percentile, median, 75% percentile, and maximum (See Source Data file for values). The red dot indicates the average number of granules in each sample. Each black dot represents the number of granules in a single field. Seven fields were acquired for each biological replicate. n = 4 biologically independent replicates. The ratio of each sample versus its experimental control was tested by a two-tailed Student’s t-test. P values are indicated. d Box plots showing the percentage of unrecovered patients-derived fibroblasts treated either with DMSO or STM-2457. The box plots are defined by minima, 25% percentile, median, 75% percentile, and maximum (See Source Data file for values). The red dot indicates the average percentage in each sample. Each black dot represents the percentage in a single field. Seven fields were acquired for each biological replicate. n = 3 biologically independent replicates. The ratio of each sample versus its experimental control was tested by a two-tailed Student’s t-test. P values are indicated. e, f Box plots illustrating the diffusion coefficients (D) of FUSP525L in living SK-N-BE cells treated with either DMSO or STM-2457 in stress or recovery conditions. The box plots are defined by minima, 25% percentile, median, 75% percentile, and maximum (See Source Data file for values). The red dot indicates the average diffusion coefficient value. Each black dot is a single measurement. n = 2 biologically independent replicates. From 16 to 26, single measurements were carried out for each condition. The ratio of each sample versus its experimental control was tested by a two-tailed Student’s t-test. P values are indicated. Source data are provided as a Source Data file.
