Fig. 10: Model for CaLB1 function in autophagy. | Nature Communications

Fig. 10: Model for CaLB1 function in autophagy.

From: Arabidopsis CaLB1 undergoes phase separation with the ESCRT protein ALIX and modulates autophagosome maturation

Fig. 10

CaLB1 is induced by salt and is necessary for resistance to salt stress. The C2 ___domain of CaLB1 binds the phospholipid PI(3)P and ATG8 proteins, which are present in autophagosomal membranes. This could form the basis of CaLB1 recruitment to autophagosomes. With the C-terminal IDR, CaLB1 binds the IDR of ALIX. CaLB1 can form molecular condensates on autophagosomes in conjunction with ALIX. The stimulation of condensate formation by CaLB1 may serve as a trigger for the formation of ALIX filaments, which in turn facilitates the subsequent recruitment and positioning of ESCRT-III filaments through multivalent interactions at the site of autophagosome closure. Together with other ESCRT-related proteins, CaLB1 and ALIX present important factors that coordinate autophagosome maturation in plant cells, especially under salt stress.

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