Fig. 4: Analogues of M-833 are resisted by M-833 resistant lines, SLI- and CRISPR-mutants. | Nature Communications

Fig. 4: Analogues of M-833 are resisted by M-833 resistant lines, SLI- and CRISPR-mutants.

From: Aryl amino acetamides prevent Plasmodium falciparum ring development via targeting the lipid-transfer protein PfSTART1

Fig. 4

A Optimised analogues of M-833: their structures, EC50 (SD) on 3D7 parasites, toxicity (CC50 (SD)) on HepG2 cells. NT = not tested. (B) EC50 values of M-833 and analogues on 3D7 and M-833 resistant parasites PopD-D7 and PopE-F10, containing the PfSTART1 mutations N309K and N330K, respectively. n = 3 biological replicates, mean +/− SD. EC50 values indicated above the bar, as well as in Supplementary Data 1. Growth curves available in Fig. S3. C EC50 values of W-991 on 3D7, CR-WT, CR-N309K and CR-N330K parasites, in the presence of 0, 0.25 or 2.5 mM glucosamine (GlcN). n = 3 biological replicates, mean +/− SD. Ordinary one-way ANOVA with Dunnett’s multiple comparison test. *p < 0.05. **p < 0.005 (CR-WT, p = 0.0265; CR-N309K, p = 0.0123; CR-N330K, p = 0.008). EC50 values and 95% confidence intervals are indicated in the table. Source data are provided as a Source Data file.

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