Fig. 7: W-991 blocks merozoite transition to ameboid ring in RBCs, and blocks transmission in mosquitoes. | Nature Communications

Fig. 7: W-991 blocks merozoite transition to ameboid ring in RBCs, and blocks transmission in mosquitoes.

From: Aryl amino acetamides prevent Plasmodium falciparum ring development via targeting the lipid-transfer protein PfSTART1

Fig. 7

A Representative images of 4D Lattice Light Sheet Microscopy of merozoites (MitoTracker; cyan) directly after invasion of RBCs (all membranes are magenta). Treatment with W-991 shows disruption to the formation of the parasitophorous vacuole membrane (magenta) compared to vehicle control. B The mean vacuole sphericity (+/− SD) for W-991 and DMSO treatments across 30 min filming period. Statistical analysis was conducted via a Nested t-test (two-tailed) in GraphPad Prism showing p = 0.716 at T = 0 mins and a p = 0.045 at T = 15 mins (*). Across three independent experiments, there were 20 and 22 events analysed for W-991 (60 nM) and DMSO (0.001%) treatments, respectively (Fig. S6 and Movie S1 and S2). C Stage V gametocytes were quantified on day 17 which demonstrates there was no defect in gametocyte development upon W-991 treatment compared to PMIX/X dual inhibitor WM382. D Oocysts were quantified in mosquito midguts on day 7 after feeding which showed a dose-dependent decrease in oocysts per mosquito with W-991 treatment. Statistical tests (two-way ANOVA) were performed in GraphPad Prism. **** indicates p < 0.0001. Positive control compound, WM832, was used at 50 nM53. Error bars indicate SD for n = 30 mosquitoes for each treatment (mean+/−SD). Data are a single representative from 3 independent experiments which can be found in Figure S7C, D. Source data are provided as a Source Data file.

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