Fig. 3: Rapid derivation of chemoresistant cells. | Nature Communications

Fig. 3: Rapid derivation of chemoresistant cells.

From: CRISPR screens reveal convergent targeting strategies against evolutionarily distinct chemoresistance in cancer

Fig. 3

a Crystal violet staining of HCT116 cells with lentiviral infection of indicated sgRNAs and oxaliplatin treatment. b–e Bar chart showing the value of IC50 of indicated cells derived from three experimental replicates shown in Supplementary Fig. 5a–d. f–i Establishment of acquired resistance to oxaliplatin in HCT116 (f) and to irinotecan in DLD1 (h) cells by “randomly” clonal selection with gradual increase of drug treatment. OXA oxaliplatin, IRI irinotecan, Mean ± SD with n = 3 biological replicates. Unpaired two-sided t test for p value. The IC50 values are shown in g and i, respectively, which are derived from three experimental replicates shown in Supplementary Fig. 5e, f.

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