Fig. 1: DYRK2 phosphorylates MYO6 at S267 in the motor ___domain in vitro. | Nature Communications

Fig. 1: DYRK2 phosphorylates MYO6 at S267 in the motor ___domain in vitro.

From: Motor ___domain phosphorylation increases nucleotide exchange and turns MYO6 into a faster and stronger motor

Fig. 1

a SDS-PAGE of GFP-tagged MYO6 immunoprecipitated from RPE cell lysate using GFP nanobodies (right) and endogenous MYO6 immunoprecipitated from A431 cell lysate using MYO6 antibodies (left). A representative image is shown of the experiment performed more than 10 times. The arrows indicate the position of GFP-MYO6 or endogenous MYO6. b MYO6 sequence peptide coverage by mass spectrometry. Identified peptides shown in shades of grey. c Sequence of the identified phosphopeptide LHLSSPDNFR for the S267 and VSLTTRVMLTTAGGTKGTVIK for the T405 and site probabilities for S266 and S267 or T405. d Predicted AlphaFold (v2.0) structure of MYO6: motor ___domain (grey), neck ___domain (green/turquoise), neck ___domain extension (gold), SAH ___domain (pink) and C-terminal cargo-binding ___domain (blue). The inset A shows the position of S267 (red) and insert-1 (yellow) and inset B highlights the position of T405 (red) in the surface loop (purple) at the actin binding site. e Conservation of MYO6 at S267 across species. f Pie charts illustrating the percentages of S267 or T405 phosphorylation in MYO6 immunoprecipitated from A431 cells grown in serum-free media (starved) or after stimulation with EGF for 15 min or phorbol 12-myristate 13-acetate (PMA) for 20 min. g Table summarising the percentages of S267 or T405 phosphorylation for MYO6 immunoprecipitated from A431 cells grown in serum-free media (starved) or after stimulation with EGF for 15 min or phorbol 12-myristate 13-acetate (PMA) for 20 min.

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