Fig. 1: TAZ is upregulated in HPV18+ cervical cancer.

A Genomic alterations of WWTR1 across human cancers determined by cBioportal analysis of TCGA data (LUSC Lung squamous cell carcinoma (n = 487), USC Uterine serous carcinoma (n = 56), ESCA Eosophageal carcinoma (n = 186), OV Ovarian serous cystadenocarcinoma (n = 579), HNSC Head and Neck squamous cell carcinoma (n = 517), CESC Cervical squamous cell carcinoma (n = 295), BLCA Bladder Urothelial carcinoma (n = 408), PRAD Prostate adenocarcinoma (n = 491), UCEC Uterine corpus endometrial carcinoma (n = 487). B Scatter dot plot analysis of WWTR1 mRNA expression against WWTR1 copy number alterations in cervical cancer determined from TCGA data (n = 278). The correlation coefficient (r) was calculated using Pearson correlation analysis. C Scatter dot plot analysis of WWTR1 mRNA expression against TAZ protein expression in cervical cancer determined from TCGA data (n = 152). The correlation coefficient (r) was calculated using Pearson correlation analysis. D Representative western blot of lysate from HPV-, HPV16+ and HPV18+ cell lines. Lysates were probed for YAP, TAZ and the loading control GAPDH (n = 3). E RT-qPCR analysis of WWTR1 or YAP1 expression in HPV-, HPV16+ or HPV18+ cell lines (n = 3). U6 transcript levels were used as a loading control. F RT-qPCR analysis of WWTR1 expression in negative, HPV16 or HPV18+ patient cervix liquid cytology samples from different CIN grades (n = 4 from each grade). U6 was used as a loading control. Maxima and minima whiskers represent the highest and lowest values, respectively. Bounds of boxes are the 75th and 25th percentiles and the centre represents the median. G Kaplan–Meier curves showing progression free survival in HPV18+ cervical cancer stratified by high or low WWTR1 expression. Survival was compared using the log-rank test. H Kaplan–Meier curves showing progression free survival in non-HPV18+ cervical cancer stratified by high or low WWTR1 expression. Survival was compared using the log-rank test. Error bars represent the mean +/- standard deviation of a minimum of three biological repeats when relevant. *P < 0.05, **P < 0.01, ***P < 0.005 (two-tailed, unpaired Student’s t-test). Source data are provided as a Source Data file.