Fig. 5: YAP and TAZ are oncogenic in HPV18 + , but not HPV16+ cervical cancer cell lines.
From: The Hippo pathway transcription factors YAP and TAZ play HPV-type dependent roles in cervical cancer
A RT-qPCR analysis of WWTR1 and YAP1 expression in TAZ, YAP or YAP/TAZ (Y/T) KD HeLa cells (n = 3). U6 was used as a loading control. B Representative western blots of TAZ, YAP and loading control GAPDH from TAZ, YAP or YAP/TAZ (Y/T) KD HeLa cell lysates (n = 3). C Growth curve analysis of TAZ, YAP or YAP/TAZ (Y/T) KD HeLa cells (n = 3). D Colony formation assay of TAZ, YAP or YAP/TAZ (Y/T) KD HeLa cells (n = 3). E Soft agar assay of TAZ, YAP or YAP/TAZ (Y/T) KD HeLa cells (n = 3). F RT-qPCR analysis of WWTR1 or YAP1 expression in TAZ, YAP or YAP/TAZ (Y/T) KD SiHa cells (n = 3). U6 was used as a loading control. G Representative western blot of YAP, TAZ and GAPDH from TAZ, YAP or YAP/ TAZ (Y/T) KD SiHa cell lysates (n = 3). H Growth curve analysis of TAZ, YAP or YAP/TAZ (Y/T) KD SiHa cells (n = 3). I Colony formation assay of TAZ, YAP or YAP/TAZ (Y/T) KD SiHa cells (n = 3). J Soft agar assay of TAZ, YAP or YAP/TAZ (Y/T) KD SiHa cells (n = 3). Error bars represent the mean +/- standard deviation of a minimum of three biological repeats. *P < 0.05, **P < 0.01, ***P < 0.005 (two-tailed, unpaired Student’s t-test). Source data are provided as a Source Data file.
