Fig. 7: RNA-seq analysis demonstrates distinct transcriptional profiles controlled by YAP and TAZ and identify TOGARAM2 as a TAZ-dependent gene.

A LogFC Heatmap of differentially expressed genes in TAZ KD (A and B KD cell lines) YAP KD (A and B KD cell lines) HeLa cell lines (n = 2). LogFC are scaled row-wise, where red and blue indicate a LogFC higher and lower than the row-wise statistical mean, respectively. B Venn diagram of DEGs upregulated and downregulated in TAZ KD (A and B) compared with NEG and YAP KD (A and B) compared with NEG, including overlapping DEGs in each comparison contrast. C Volcano plot of TAZ KD RNA-sequencing in HeLa cells defined significant at adjusted P-value < 0.05 and logFC threshold at 0.5. Non-significantly altered genes are highlighted black and grey in A1 and A2 contrasts, respectively. Significantly upregulated genes are highlighted dark green and light green in A1 and A2 contrasts, respectively. Significantly downregulated genes are highlighted dark red and light red in A1 and A2 contrasts, respectively. DEGs significantly altered in TAZ KD only are highlighted blue. D Counts per million of TOGARAM2 expression in RNA-sequencing of NEG, TAZ KD (A and B KD cell lines) and YAP KD (A and B KD cell lines) (n = 2). E RT-qPCR analysis of TOGARAM2 expression in HPV-, HPV16+ or HPV18+ cell lines (n = 4). U6 transcript levels were used as a loading control. F RT-qPCR analysis of TOGARAM2 expression in negative, HPV16 or HPV18+ patient cervix liquid cytology samples from CIN grades (n = 4 from each grade). U6 was used as a loading control. Maxima and minima whiskers represent the highest and lowest values, respectively. Bounds of boxes are the 75th and 25th percentiles and the centre represents the median. G Graph showing correlation between TOGARAM2 and WWTR1 expression from C. The correlation coefficient (r) was calculated using Pearson correlation analysis. H, I Kaplan–Meier curves showing overall survival in cervical cancer stratified by HPV18+ or non-HPV18+. Survival was compared using the log-rank test. Errors represent the mean +/- standard deviation of a minimum of three biological repeats. *P < 0.05, **P < 0.01, ***P < 0.005 (two-tailed, unpaired Student’s t-test). Source data are provided as a Source Data file.