Fig. 4: EGO-1 and its interacting partners define the E granule.
From: Germ granule compartments coordinate specialized small RNA production
a Pachytene germ cells of animals that express EGC-1::GFP and the indicated tagRFP- or mCherry- tagged proteins. All images are representative of more than three animals. b Quantification of colocalization between the indicated fluorescent proteins of pachytene germ cells. Each data point represents the Pearson’s R-value showing the degree of colocalization between two fluorescence channels covering an individual germ cell (15 germ cells in total from 3 independent animals). The solid black line indicates the mean value. PGL-3 is a constitutive component of the P granule at all stages of development19. Source data are provided as a Source Data file. c Summary of the perinuclear localization of EGO-1 and EGO-1 interactors. Note that E granules disintegrates during diakinesis. The green and pink balls represent E granules and Mutator foci, respectively. d Representative pictures of germ granules in pachytene germ cells showing that the E granule is nonrandomly positioned within the germ granule. A total of 210 germ granules from 7 worms were analyzed. The percentage of sequential assembly of E, P and Z granules was counted. All images were taken by the Leica THUNDER imaging System and deconvoluted using Leica Application Suite X software (version 3.7.4.23463).
