Fig. 5: EGC-1 and ELLI-1 are required for the accumulation of the EGO module in the E granule.
From: Germ granule compartments coordinate specialized small RNA production
a Pachytene germ cells of animals that express EGC-1::GFP or ELLI-1::GFP in the indicated animals. The drh-3(ne4253) lesion (T834M) alters a residue that contacts RNA in the Vasa crystal structure40. The hypomorphic drh-3(ne4253) allele was reported to cause RNAi defective phenotype, siRNA biogenesis defects and dramatically reduced brood size at 20 °C40. The indicated animals were grown at 20 °C. b, c Western blotting of the indicated GFP-tagged proteins in the indicated animals. Actin was used as a loading control. Each experiment has been repeated three times with similar results. Source data are provided as a Source Data file. d Pachytene germ cells of animals that express GFP::EGO-1, EKL-1::GFP or DRH-3::GFP in the indicated animals. The perinuclear localization of EGO-1, EKL-1 and DRH-3 are dramatically decreased upon the loss of EGC-1 or ELLI-1 and are completely blocked in egc-1;mut-16 or elli-1;mut-16 double mutant animals. e, f, g Western blotting of the indicated GFP-tagged proteins in the indicated animals. Actin was used as a loading control. Each experiment has been repeated three times with similar results. Source data are provided as a Source Data file. Defects in E granule assembly did not affect the expression levels of the EGO module components (EGO-1, DRH-3 and EKL-1). h Fluorescence micrographs of the indicated animals expressing GFP::EGO-1 and tagRFP::ELLI-1. i Fluorescence micrographs of pachytene germ cells from animals expressing GFP::ZNFX-1, mCherry::MUT-16, SIMR-1::tagRFP and PGL-1::GFP in the indicated animals. All images were taken by a Leica upright DM4 B microscope equipped with a Leica DFC7000 T camera and an HC PL APO 100x/1.40-0.70 oil objective. All images are representative of more than three animals.
